Table 2.
Developmental rate of embryos following 3 h incubation in PBSs with various pH, and HTF followed by culture for 120 h in appropriate conditions
| pH | No. of embryos | Development (%) | ||
|---|---|---|---|---|
| Morulae at day 3 | Expanded blastocyst at day 4 | Hatching and hatched blastocyst at day 5 | ||
| HTF (7.3) | 42 | 33 (78.6) | 30 (71.4) | 25 (59.5) |
| 6.2 | 36 | 15 (41.7)a | 18 (50) | 12 (33.3)a |
| 6.6 | 40 | 22 (55) | 26 (65) | 20 (50) |
| 7 | 40 | 31 (77.5) | 24 (60) | 20 (50) |
| 7.35 | 42 | 37 (88.1)b | 30 (71.4) | 31 (73.8)b |
| 7.6 | 39 | 27 (69.2) | 25 (64.1) | 20 (51.3) |
| 8 | 43 | 23 (53) | 21 (49) | 17 (39) |
Experiments were replicated seven times with at least five morphologically normal embryos in each group. In each column figures with different superscripts are statistically different; P < 0.05