Table 2.
Numbers of TE and ICM cells in hatched blastocysts grown in vitro for 5 days
| Stage of vitrification | Hatched Blastocysts(n) | TE (mean ± SD) | ICM (mean ± SD | TCN (mean ± SD) | Ratio ICM/TE (mean ± SD) |
|---|---|---|---|---|---|
| 2-cell control | 33 | 67.27 ± 9.36 | 36.24 ± 9.21 | 103.52 ± 12.82 | 0.55 ± 0.15 |
| Vitrified 2-cell | 30 | 54.97 ± 10.48a | 31.10 ± 8.79a | 85.40 ± 17.02a | 0.57 ± 0.13 |
| 4-cell control | 38 | 68.63 ± 11.32 | 36.37 ± 8.57 | 105.00 ± 17.79 | 0.53 ± 0.10 |
| Vitrified 4-cell | 30 | 51.40 ± 10.06a | 29.60 ± 7.06a | 81.00 ± 13.02a | 0.59 ± 0.16 |
| 8-cell control | 37 | 67.89 ± 9.66 | 37.46 ± 7.53 | 105.57 ± 15.00 | 0.55 ± 0.08 |
| Vitrified 8-cell | 34 | 53.44 ± 10.18a | 31.50 ± 7.23a | 84.94 ± 13.75a | 0.60 ± 0.16 |
Means of total cell numbers (TCN), inner cell mass (ICM) and trophectoderm (TE) of blastocysts fixed and stained with propidium iodide and ethanol/Hoechst. Values are represented as mean ± standard deviation
acompare to control group, P < 0.001