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. 1998 Sep 1;95(18):10920–10925. doi: 10.1073/pnas.95.18.10920

Figure 4.

Figure 4

Identification of the C/EBPδ-binding site in the NGF promoter. (A) DNase I footprinting analysis of the NGF promoter with recombinant C/EBPδ. The labeled fragment NGF−615+50 was incubated in the presence (+) or absence (−) of bacterially expressed C/EBPδ and partially digested with DNase I. Lanes G and A+G correspond to Maxam–Gilbert sequencing reactions performed on the same probe. Sequence of the protected area is shown. (B) Transactivation of 5′ deletion mutants of the NGF promoter by C/EBPδ in CTX-TNA2 cells. Each mutant was cotransfected with either pMEX or pMEX-C/EBPδ expression vectors and pRSV β-galactosidase reporter vector. The luciferase activity was measured 48 hr after transfection and normalized to β-galactosidase activity. Data, expressed as percentage of the full-length construct (NGF−615+50 with pMEX), are the mean ± SEM of three separate experiments, with two independent samples in each experiment. ∗, P < 0.01 vs. pMEX (ANOVA and Dunnett’s test).