Table 5.
In vitro percentage adsorption of ZON by different mineral clays, humic substances and yeast cell-wall derived products as assessed by chemical and biological methods*
| Producta | 5 mg/ml | 2.5 mg/ml | 1 mg/ml | |||||
|---|---|---|---|---|---|---|---|---|
| HPLCb | HPLC | BIOASSAYc | HPLC | |||||
| Ac | Ak | Ac | Ak | Ac | Ak | Ac | Ak | |
| Mineral1 | 92±3 | 88±6 | 71±9 | 74±2 | 83±2 | 74±3 | 63±10 | 43±1 |
| Mineral2 | 36±4 | 6±11 | 8±7 | 4±7 | 5±6 | 1±2 | 7±9 | 2±2 |
| Mineral3 | 47±11 | 15±11 | 8±7 | 4±7 | 25±15 | 7±14 | 13±5 | 5±4 |
| Humic1 | 88±2 | 67±3 | 69±7 | 68±10 | 66±2 | 56±5 | 61±3 | 57±9 |
| Humic2 | 90±1 | 88±4 | 69±13 | 65±12 | 78±15 | 78±13 | 68±2 | 61±5 |
| Humic3 | 48±9 | 16±5 | 38±2 | 13±11 | 33±15 | 21±17 | 22±5 | 17±1 |
| Humic4 | 98±8 | 95±2 | 94±3 | 92±1 | 87±10 | 90±11 | 56±11 | 47±15 |
| Humic5 | 9±8 | 38±15 | 7±8 | 15±1 | 15±6 | 17±6 | 10±12 | 17±3 |
| Humic6 | 9±8 | 6±12 | 2±4 | 14±2 | 11±8 | 20±4 | 4±4 | 7±13 |
| Yeast 1 | 71±5 | 68±6 | 55±8 | 48±1 | 68±8 | 52±12 | 46±23 | 30±5 |
| Yeast 2 | 67±1 | 59±7 | 50±7 | 39±4 | 58±13 | 39±15 | 39±11 | 27±5 |
| Yeast 3 | 88±5 | 77±1 | 69±11 | 55±4 | 85±16 | 72±11 | 63±7 | 33±9 |
| Yeast 4 | 3±4 | 6±5 | 0 | 2±3 | 30± 13 | 69± 17 | 0 | 4±5 |
| Charcoal | 100 | 100 | 99±1 | 100 | 100 | 100 | 97±1 | 98±2 |
*Values are means±SD of three independent experiments.
Ac = acidic adsorption.
Alkaline = alkaline adsorption.
aAll products included at the three indicated concentrations: 5, 2.5 and 1 mg/ml;
b Detection limit: 0.04mg/l;
cCell Proliferation Bioassay.