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. 2009 Aug 19;29(33):10350–10356. doi: 10.1523/JNEUROSCI.2339-09.2009

Figure 2.

Figure 2.

Overexpression of human calpastatin in rat cortical cultures prevents ionomycin-induced NaCh proteolysis. A, Representative Western blots showing the effect of calpastatin on NaCh proteolysis. At DIV 19, 10 d after transduction using an AAV with EGFP alone or EGFP and human calpastatin, cortical cultures were incubated with ionomycin (1 μm) for 1 h. Cultures transduced with calpastatin/EGFP displayed no significant ionomycin-induced NaCh α-subunit (pan, Nav1.2 C terminal) or spectrin proteolysis compared with those transduced with EGFP alone. B, Quantification of the representative blots seen in A. Data normalized to the corresponding untreated EGFP control (n = 4, *p < 0.05 from the EGFP control).