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. 2009 Dec 10;106(52):22534–22539. doi: 10.1073/pnas.0908718106

Fig. 2.

Fig. 2.

TCP and miR319 expression. (A) Mature miRNA sequence of three miR319 genes in Arabidopsis: miR319a, miR319b, and miR319c. miR319a and miR319b encode the identical miRNA. The mutation (G to A) in miR319a129 is indicated by the arrow above the miR319a sequence. The miR319 genes target a subset of TCP transcription factors such as TCP4. The tcp4soj6 mutation (C to T) is indicated by the arrow below the TCP4 sequence. The tcp4soj6 and miR319a129 mutations are complementary. (B) Whole-mount miR319c:GUS seedling. The base of young leaf primordia is strongly stained. (C) Section through a miR319c:GUS seedling. The base of young leaf priordia is strongly stained. (D) Whole-mount miR319a:GUS seedling. Stipules are strongly stained. (E) Section through miR319a:GUS seedling. Stipules are strongly stained. (F) Section through two miR319c:GUS flowers. In the stage 5 flower (Right), the pedicel and base of organ primordia are stained. In the stage 8 flower (Left), the petals, stamen filaments, and the base of the carpels and sepals are stained. Flowers staged according to Smyth et al. (25). (G) Section through a stage 10 miR319a:GUS flower. The proximal region of the petals is stained. (H) Section through a stage 8 miR319a:GUS flower. The petals are strongly stained. (I) Section through three miR319a:GUS flowers. In the stage 5 flower (Center) GUS activity is detectable at the base of the sepals and throughout developing second, third, and fourth whorl organs. In the stage 6 (Left) and stage 7 (Right) flowers, GUS activity is detectable at the base of the sepals and carpels, and at high levels throughout developing petals. In stamens, higher levels of GUS activity are detected in the stamen filament primordia compared to anther primordia. (J) Whole-mount miR319b:GUS inflorescence. The sepal and stamen abscission zones of late stage flowers are stained. (K) qRT-PCR demonstrates that TCP2, TCP3, TCP4, TCP10, and TCP24 RNA levels increase between 1.4- and 2.5-fold in inflorescences from miR319129 mutants as compared to wild-type. TCP genes are down-regulated in jaw-D inflorescences. Error bars, SEM. All values are significantly different from wild-type. P values for TCP2, TCP3, TCP4, TCP10, and TCP24 are 0.000, 0.028, 0.006, 0.029, and 0.029 respectively for miR319a129 and 0.026, 0.003, 0.000, 0.001, and 0.003 respectively for jaw-D. *, P < 0.05, ‡, P < 0.005.