Figure 3.

Graphs showing the effects of NGF or CytC on glutamate levels in the rat hippocampus. Samples were collected bilaterally every 20 min, and glutamate in the effluent was measured by HPLC. The values of the last samples after equilibration were designated as baseline (100%) at time zero (0) on both sides, and all other values were expressed as percentages of these values. Expressed in this manner, the baseline values did not differ significantly from the values of the previous two samples. Immediately after the samples were collected at time 0, the dialysis perfusates on one side were switched to either NGF or Cyt (0.01 μg/μl). Neither NGF (n = 8) nor CytC (n = 6) had a statistically significant effect on glutamate levels (ANOVA).