Fig. 3.

Subsets of glutamatergic, somatostatin, CRH, nitric oxide, cholinergic, and L-dopa neurons require Math1. (A) Schematics of hindbrain nuclei containing Math1 lineages (blue, listed to right) on coronal hemisections, rostral to caudal (levels 1–6, Fig. 1B). (B–G) Magnified boxed regions in A from corresponding E18.5 WT and Math1-null sections stained with ISH probes for glutamate (Vglut2), somatostatin (SST: Sst), corticotropin releasing hormone (CRH: Crh), nitric oxide (NO: Nos1), acetylcholine (ACh: Vacht), and levodopa (L-Dopa: tyrosine hydroxylase, Th) neurons. Nuclei containing Math1-dependent Vglut2 or Sst are marked by dotted outlines, whereas other Math1-dependent neurotransmitters are marked by gray arrowheads. Abbreviations: Please see Table 1 for list of nuclei. (Scale bar: B–G, 500 μm.)