Fig. 4.

Nitric oxide, CRH, and somatostatin neurons of the arousal, interoceptive, and conscious proprioceptive systems arise from the RL. (A) Schematic of coronal hemisection with nuclei containing Math1 lineages (blue); boxed region magnified above shows the LDT and BN (green) medial to the LC (orange). (B and C) Dotted outlines mark approximate boundaries of these three nuclei on adjacent sections from a Math1^Cre*PR/+;ROSA^EYFP/+ hindbrain (E18.5) induced at E10.5 and stained for EYFP (green) and Nos1 (red) with either Crh (B) (blue) or Th (C) (blue). (B′ and C′) Regions magnified from yellow boxes in A and B with yellow dashed boxes further magnified below each. EYFP colabeled with Crh and Nos1 but not with Th. (D) Schematic showing nuclei containing Math1 lineages (blue) on a coronal hemisection from the caudal medulla. (E) Boxed region from D shows EYFP and Sst expression in the Cu/Gr nuclei. Dashed box, magnified below, shows colabeling of EYFP (somas) with Sst (cytoplasmic + extracellular). Abbreviations: CRH, corticotropin releasing hormone; Nos1, nitric oxide synthase 1; Sst, somatostatin. See Table 1 for other abbreviations. (Scale bar: B and C, 250 μm; B′, C′, and E′, 80 μm; Lower frames of B, C, and E, 40 μm.)