Fig. 2.

Expression of transgenic htt in HD mouse brains. (A) Comparison of mutant htt staining density between GFAP-HD and N171-82Q mouse brain cortex. The front cortex region was stained with 1C2 at a 1:3,000 dilution. Smaller nuclear size with htt labeling in glial cells and reactive astrocytes (arrow) are seen in GFAP-HD mouse brain, whereas N171-82Q mouse brain shows more abundant and larger neuronal nuclear staining as well as small neuropil aggregates. (Scale bar, 20 μm.) (B) High-magnification (×63) micrographs of brain sections. (Scale bar, 10 μm.) Brain sections were stained with 1C2. (C) Double immunostaining of GFAP-HD mouse brain with mouse antibody 1C2 for mutant htt (red) and rabbit antibody for GFAP (green). The merged image also shows nuclear staining by Hoechst dye (blue). (Scale bar, 10 μm.)