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. 2009 Dec 17;106(52):22239–22244. doi: 10.1073/pnas.0903750106

Fig. 1.

Fig. 1.

The 70S ribosome and RNC constructs. Left: schematic diagram of the 70S ribosome with the 30S and 50S subunits highlighted in red and blue, respectively. The PTC is shown occupied by tRNA (purple). Mapped onto the ribosome is the NC construct as produced via the pLDC vectors. From the C terminus at the PTC is the 17-residue SecM motif, (yellow), followed by the selected NC (blue). The NC is shown fused to a hexa-His tag; Center: the general features of the pLDC-17 vector are shown (above) in the N to C direction. Schematics of the two RNCs described in this study are shown below: ddFLN646–839-RNC is Dom5 plus a 90 residue sequence derived from Dom6 fused to SecM; ddFLN646–750-RNC is Dom5 alone fused to SecM; Right: representation of the two domain structure (from 1QFH.pdb) of Dom 5 and Dom 6 (ddFLN646–857). The ddFLN646–839-RNC vector comprises an NC construct designed such that the final strand in Dom6 (G-strand, shaded gray) is absent.