Fig. 1.

TIR1/AFB gene expression involves posttranscriptional regulation. GUS expression in the cotyledons, young leaves (A–H), primary root tip, and young lateral roots (I–X) of 8-day-old transgenic seedlings. X-Gluc staining is representative of expression levels in seedlings containing pTIR1:GUS (A, I, and J), pTIR1:cTIR1:GUS (B, K, and L), pAFB1:GUS (C, M, and N), pAFB1:cAFB1:GUS (D, O, and P), pAFB2:GUS (E, Q, and R), pAFB2:cAFB2:GUS (F, S, and T), pAFB3:GUS (G, U, and V), and pAFB3:cAFB3:GUS (H, W, and X). Samples A–H, J–L, N–P, and R–T X-Gluc were stained overnight. Samples I, M, Q, and U–X were stained for 5 h.