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. 2009 Dec 16;106(52):22540–22545. doi: 10.1073/pnas.0911967106

Fig. 2.

Fig. 2.

miR393 influences the auxin response in the root. (A) Four-day-old seedlings grown on MS media were transferred to media containing 2,4-D. Root elongation was measured after an additional 4 days and expressed as a proportion of growth in the absence of auxin (Scale bars, SE). (B) Lateral root assay. Four-day-old seedlings grown on MS media were transferred onto media −/+ 2,4-D for an additional 9 days. The number of emerged lateral roots/mm primary root length was measured. (Scale bars, SE.) (C–E) Expression of pmiR393:GUS in the primary root tip (C) and lateral roots (D and E) of 10-day-old seedlings. (F) Expression of pTIR1:cTIR1:GUS in an emerging lateral root in a 10-day-old seedling. (G–I) Expression of pimR393a:GFP in the primary root tip region (G, arrow), root elongation zone (H), and beneath an emerging lateral root (I, asterisk) in 10-day-old seedlings.