Fig. 1.

DHX29 is a cytoplasmic protein that associates with the 40S ribosomal subunit. (A) Distribution of DHX29 in 10%–50% sucrose density gradient fractions of HeLa S3 cells as measured by immunoblotting. Polysomal profile distributions of eIF3b, eIF3g, rpS6, rpL6, and β-actin are shown for comparison. DHX29#1 and DHX29#2 represent antibodies against DHX29, from Bethyl Laboratories and Santa Cruz Biotechnologies, respectively. (B) Immunofluorescent detection of HA-DHX29 protein in HeLa cells using an anti-HA antibody (α-HA).