Fig. 2.

NUMB expression and Notch signaling in NSCLCs. (A and B) Inverse correlation between NUMB and activated-NOTCH-1 status in NSCLCs. (A) Representative IHC in serial sections (original magnification ×40). (B) Quantitative assessment of activated-NOTCH-1 in NSCLC as a function of NUMB-class. (C) HES1 mRNA levels in NSCLCs displaying different levels of NUMB. Data were obtained by Q-PCR on frozen specimens of the cohort of 49 patients and are expressed relative to the reference cell line BEAS-2B (= 1). The six patients harboring mutations in the NOTCH-1 gene (see Figs. 3A and 5A) were excluded from the results reported in panels (B) and (C). (D) NUMB-class (measured by IHC) and HES1 levels, measured as in (C), in 10 primary cultures from NSCLC patients.