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. 2009 Dec 10;106(52):22293–22298. doi: 10.1073/pnas.0907781106

Fig. 6.

Fig. 6.

Inhibition of Notch signaling affects the growth potential of primary NSCLC cultures harboring alterations of the Notch pathway. A. Eight NSCLC primary cultures, representing 3 groups based on NOTCH-1/NUMB status, were tested for their sensitivity to the GSIs MRK-003 and DAPT. The characteristics of the primary cultures are summarized in the panel. (B and C) Survival of the primary NSCLC cultures, after treatment with MRK-003 (B) or DAPT (C) with respect to vehicle (DMSO). Results are expressed as the mean ± SEM of two independent experiments. (D) Summary of GSI experiments. Survival, with respect to vehicle-treated cells, is shown for the three types of NSCLC cultures described in (A): filled bars, “WT” cells (no alterations in the Notch pathway, see main text); empty bars, NUMB-negative cells; gray bars, NOTCH-1-mutated cells. Results for primary cultures belonging to the same group were combined and are expressed as the mean ± SEM of two independent experiments. Asterisks indicate a significant difference (P < 0.01) relative to vehicle-treated cells as well as to WT cells treated with MRK-003 (left) or DAPT (right).