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. 2009 Nov 11;20(12):1652–1664. doi: 10.1089/hum.2009.012

FIG. 3.

FIG. 3.

Stimulation of OVA-specific CD8+ and CD4+ T cell responses in vitro by murine bone marrow-derived dendritic cells (BMDCs) modified by nonintegrating lentiviral vector encoding OVA antigen. (A–D) CD8+ and CD4+ T cells specific for OVA were collected from the spleens of OT1 and OT2 TCR transgenic mice and cocultured in vitro with FKOVA/VSVG(IN+)- or FKOVA/VSVG(IN–)-modified BMDCs for 3 days. FUW/VSVG(IN+)-transduced BMDCs served as the negative control. BMDCs pulsed with OVAp1 (SIINFEKL) or OVAp2 (ISQAVHAAHAEINEAGR), which are recognized by OT1 T cells and OT2 T cells, respectively, were included as positive controls. (A and B) OT1 and OT2 T cells were cocultured with various ratios of BMDCs to OT1 or OT2 T cells in vitro for 3 days. The culture supernatant was measured for the secretion of IFN-γ by ELISA. (C and D) An 3H incorporation assay was used to measure the proliferative activities of (A) treated OT1 T cells and (B) OT2 T cells.