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H2O2 sensitivity of the B. japonicum WT and the C183A-FixK2-expressing mutant strain 8854. Soft agar (0.9%) plates with PSY medium were inoculated with the indicated B. japonicum strains. Exposure to H2O2 occurred by radial diffusion from the paper disks (placed on top) that had been soaked with the indicated H2O2 concentrations. Disks at the bottom contained H2O for control.
