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Multiple conformations exist within a single crystal structure. The structure and electron density is of arPTE 4M (3A4J). 2Fo-Fc density is colored blue (contoured at 1.5 σ); Fo-Fc density is colored green (contoured at 3.0 σ). This shows residues R254, Y257, L271, and F272 adopt a secondary open conformation on one side of the active cleft, whereas F132 and T173 also adopt a secondary open conformation on the opposite side.
