Table 1.

Kinetic parameters, activation energy (Ea), and activation entropy (ΔS^‡) of the four enzymes studied

Enzyme	kcat s−1	Km μM	kcat/Km s−1 M−1	Ea (kJ mol)	ΔS‡ (J/K mol)
pdPTE	1,600 ± 50	95 ± 10	1.68 × 107	39.9	−60.1
pdPTE R254	410 ± 20	27 ± 3	1.52 × 107	25.7	−119.1
arPTE	3,180 ± 100	190 ± 19	1.67 × 107	31.2	−79.9
arPTE 4M	4,660 ± 140	216 ± 13	2.16 × 107	32.1	−74.0
arPTE 8M	1,510 ± 20	148 ± 5	1.02 × 107	18.8	−127.7