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Actin-activated ATPase activities of wild-type and loop 3 mutants of Chara myosin motor domain. Values are averages of 3 measurements for each mutant preparation. Vmax and Kapp were determined by fitting data to the Michaelis-Menten equation. Averaged Vmax and Kapp values from 2 independent preparations were shown in Table 3. Because the activity of L3 (-5) increased almost linearly with the increase in actin concentration, both Vmax and Kapp values of L3 (-5) could not be determined.
