Table 3.
Sequences, net charge and activity of wild type and loop 3 mutants of Chara myosin motor domain
| Sequence* | Net charge | Vmax (Pi/head/s)† | Kapp (μM)† | Velocity (μm/s)§ | |
|---|---|---|---|---|---|
| L3 (−5) | AAAFAA | 0 | ND‡ | ND‡ | 3.3 ± 0.2¶ |
| L3 (N-3) | AAAFKR | +2 | 260 | 81 | 4.3 ± 0.3 |
| L3 (C-2) | KHKFAA | +3 | 540 | 44 | 4.5 ± 0.2 |
| WT | KHKFKR | +5 | 580 | 23 | 4.8 ± 0.2 |
| L3 (+2) | KKKHKFKR | +7 | 390 | 12 | 4.3 ± 0.2 |
*Positively charged amino acid and altered amino acid are shown in bold and italic, respectively.
†Values are averages of two measurements on two independent preparations.
‡We could not determine Vmax and Kapp of L3 (−5) because activity increased almost linearly (see Fig. 2).
§Values are averages ± SD of 40–50 actin filaments on two independent preparations.
¶Velocity of L3 (−5) was done in the presence of 0.8% methylcellulose.