Fig. 3.

Location of the crcZ gene (open arrow) between the cbrB and pcnB genes (gray arrows) in P. aeruginosa. The existence of an inversely oriented sRNA gene, P30 (open arrow with dashed lines), has been published (26). ρ-independent terminators are indicated by stem–loop structures. The deletions of the putative P30 sequence (in strain PAO6677) and of the crcZ promoter region (in PAO6679) are indicated by underlining the nucleotide sequence. The nucleotide sequence of crcZ is shown in boldface, and its σ⁵⁴-promoter (-12 and -24 boxes) is shown in boldface and boxed. The five CA motifs are boxed. A possible ρ-independent terminator of P30 is indicated by convergent arrows placed above the sequence. The 3′ end of the in vitro transcribed CrcZ′-RNA used for the band shift experiment (Fig. 6 and Fig. S6) is indicated by an asterisk.