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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Dev Biol. 2009 Oct 20;337(1):63–73. doi: 10.1016/j.ydbio.2009.10.003

Fig. 11. The GATA4-expressing EBs eventually are highly enhanced for liver markers.

Fig. 11

The EBs derived entirely from iGATA4ES cells were induced to express Gata4 at day 2, plated at day 10 under conditions that normally support mesoderm development, and harvested at day 17. Shown are representative qPCR results analyzing cDNA isolated from induced EBs compared with levels of transcripts (as indicated) in the uninduced control cells. The relative levels are plotted with the data log2 transformed. The GATA4-expressing cells have relatively high levels of transcripts for liver markers Afp and Alb, and relatively unchanged levels for the pancreas markers Pdx1 and Ins. Multiple independent experiments gave equivalent results.