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. 2010 Jan 7;16(1):21–29. doi: 10.3748/wjg.v16.i1.21

Figure 1.

Figure 1

Phenotypic and functional analysis of DC derived from MLN and LP of healthy mice. A: Primary DC were isolated from MLN (MLN-DC) and colonic LP (LP-DC) of healthy mice. CD11c+ DC were stained with FITC-conjugated mAbs for expression of MHC-class II and the costimulatory molecules CD40, CD80 and CD86; B: Isolated DC from spleen, MLN and LP were incubated for 24 h in complete medium partly stimulated with 5 μg/mL CpG (5 wells per situation). Cytokine levels were measured within the supernatant by ELISA. aP < 0.05. Data presented are representative of three independent experiments.