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. 2009 Dec 15;23(24):2887–2899. doi: 10.1101/gad.1865909

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Copyright © 2009 by Cold Spring Harbor Laboratory Press

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Figure 3. — Fin1 associates with 14–3–3, outer kinetochore proteins, and PP1. (A,B) MS analysis identified Fin1-binding proteins. Fin1-Flag protein was purified from 5 L of asynchronously growing cells (SBY5962). (A) Sample was analyzed by SDS-PAGE followed by silver staining. Two proteins (36 kDa and 34 kDa) specifically copurified with Fin1-Flag. (B) Purified sample was analyzed by LC-MS/MS, and a summary is shown in the table. Outer kinetochore proteins are shown in bold. (C) Coimmunoprecipitation experiments confirmed that Fin1 specifically associates with 14–3–3, Ndc80, and the Glc7 phosphatase. Proteins were purified with anti-Flag antibodies from cells containing Bmh2-Myc and Glc7-HA that express either Fin1-Flag (SBY6368) or untagged Fin1 (SBY6370). Samples were analyzed by immunoblots with the corresponding antibodies.