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. 2009 Dec 15;23(24):2887–2899. doi: 10.1101/gad.1865909

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Copyright © 2009 by Cold Spring Harbor Laboratory Press

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Figure 4. — Fin1 partially mediates the interaction between Glc7 and kinetochore proteins. (Top) Ndc80-Myc was immunoprecipitated from fin1Δ (SBY7895) and FIN1 (SBY7897) cells expressing Glc7-HA. Purified samples were analyzed by immunoblots using anti-Myc and anti-HA antibodies. Glc7-HA cells with untagged Ndc80 (SBY625) were used as negative control. (Bottom) Dsn1-Flag was purified with anti-Flag antibodies from fin1Δ (SBY7899) and FIN1 (SBY7900) cells expressing Glc7-HA. Purified samples were analyzed by immunoblots using anti-Flag and anti-HA antibodies. Note that the Dsn1-Flag band overlaps with a background signal in the input.