Figure 4.

An in vitro RNAi screen to identify functionally relevant miR-19 targets. (A) List of the genes assayed in the in vitro shRNA screen. (B) Scatter plot summarizing the result of the screen. Each dot represents an individual shRNA construct. The X-axis is the percentage of GFP cells at the beginning of the experiment (2 d after infection) and the Y-axis is the percentage of GFP cells 11 d later. The green dot identifies the empty vector control. shRNAs that scored positive in the screen are highlighted in red and labeled. Dots corresponding to genes for which at least two out of three shRNAs provided significant growth advantage are labeled. PIG-miR-19a/b was included in the screen as positive control (blue dot).