Figure 1.

Receptor- and G_i/o-involvement in Noci-induced flexor responses. (A) A representative trace of Noci-induced flexor responses. Noci (1 fmol) was given i.pl. consecutively every 5 min, as indicated by the arrow. (B) Effects of Noci-R antisense and mismatch oligodeoxynucleotides on Noci- or SP-induced responses at various doses. Results were expressed as % of maximal reflex. (C) Effects of PTX on Noci-induced responses. PTX (10 ng) was given 5 min after the second challenge of Noci as a control. (D) Dose-response curves for Noci or SP in the presence or absence of PTX (10 ng)-treatments. (A–C) Treatments as indicated in the figure were compared with agonist treatment alone. *P < 0.05. Other details were as described under Materials and Methods. Each point represents the mean ± SEM from separate five experiments.