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. Author manuscript; available in PMC: 2010 Dec 9.
Published in final edited form as: J Am Chem Soc. 2009 Dec 9;131(48):17677–17689. doi: 10.1021/ja907852c

Figure 3.

Figure 3

In vitro assay of SsfL1 activity and substrate specificity. (A) Synthesis of salicylyl-AMP as indicated by the release of PPi in the presence of ATP, salicylate 19 and the salicylyl-CoA ligase SsfL1. i) all reaction components; ii) no 19; iii) no ATP; iv) no SsfL1; and v) pyrophosphate reagent only. (B) Utilization of substituted benzoic acids by SsfL1 as indicated by PPi release assay. The structures of compounds indicated by number here are shown in Scheme 3.