Table 1.
Thermodynamic dissociation parameters of single base bulge-containing duplexes relative to the parent dodecamer and 13-mer duplexa
| Duplex acronym | Tm (°C) | ΔG° (kcal/mol) |
 (kcal/mol) |
TΔS° (kcal/mol) |
|---|---|---|---|---|
| CAC/GG | 57.9 | 14.3 | 86.9 | 72.6 |
| CTC/GG | 55.0 | 12.3 | 78.5 | 66.2 |
| CCC/GG | 59.2 | 13.3 | 81.7 | 68.4 |
| CGC/GG | 52.4 | 7.4 | 45.5 | 38.1 |
| GAG/CC | 58.5 | 14.1 | 85.7 | 71.6 |
| GTG/CC | 59.1 | 14.9 | 90.7 | 75.8 |
| GCG/CC | 55.6 | 9.8 | 58.8 | 49.0 |
| GGG/CC | 59.8 | 14.5 | 86.0 | 71.5 |
| GG/CC | 68.0 | 17.5 | 94.3 | 76.8 |
| GTG/CAC | 69.0 | 19.0 | 104.1 | 85.1 |
aThermodynamic parameters are reported at 25.0°C following extrapolation assuming ΔCp = 0. Standard deviations for Tm, ΔG°, ΔH° and TΔS° are within 0.1°C, 0.2, 1.0 and 1.0 kcal/mol, respectively.