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. 2009 Oct 23;38(1):253–264. doi: 10.1093/nar/gkp766

Figure 2.

Figure 2.

Expression profile of CELF proteins in the two cell models. (A) Total protein was isolated from CA77 (labeled as C) and HeLa (labeled as H) cells and western blot analysis was performed using antibodies against CUG-BP1 or ETR-3 and U1 70K as a loading control. (B) Total RNA was isolated from CA77 and HeLa cells and RT–PCR was performed using oligonucleotides designed to anneal to CELF family members 3–6 in regions conserved between rat and human. PCR amplification products are 201 nucleotides for CELF3, 240 nucleotides for CELF4, 241 nucleotides for CELF5, and 205 nucleotides for CELF6. β-actin was used as a control, and has a PCR amplification product of 376 nucleotides. The human neuroblastoma cell line, SK–N–SH, was used as a control to verify that all of the oligonucleotides worked in both human and rat cells.