Table 1.
Sequences of the nucleic acids used in this studya
| Name | Sequence |
|---|---|
| Template A (TA) | 5′-AGT AAC GGC AGA CTT CTC CTA AGG AGT CAG GTG CAC CAT G-3BioTEG-3′ |
| Template T (TT) | 5′-AGT AAC GGC AGA CTT CTC CTT AGG AGT CAG GTG CAC CAT G-3BioTEG-3′ |
| Template G (TG) | 5′-AGT AAC GGC AGA CTT CTC CTG AGG AGT CAG GTG CAC CAT G-3BioTEG-3′ |
| Template C (TC) | 5′-AGT AAC GGC AGA CTT CTC CTC AGG AGT CAG GTG CAC CAT G-3BioTEG-3′ |
| Probe 1 A (P1A) | 5′-TGC ACC TGA CTC CTA-3′ |
| Probe 1 T (P1T) | 5′-TGC ACC TGA CTC CTT-3′ |
| Probe 1 G (P1G) | 5′-TGC ACC TGA CTC CTG-3′ |
| Probe 1 C (P1C) | 5′-TGC ACC TGA CTC CTC-3′ |
| Probe 2 (P2) | 5′-5Phos-AGG AGA AGT CTG CCG-3′ |
| Probe 2 without P04 | 5′-AGG AGA AGT CTG CCG-3′ |
| Probe 1Gap1 | 5′-TGC ACC TGA CTC CT-3′ |
| Probe 1Gap2 | 5′-TGC ACC TGA CTC C-3′ |
| Probe 1lnsertA | 5′-TGC ACC TGA CTC CTG A-3′ |
| Probe 1lnsertT | 5′-TGC ACC TGA CTC CTG T-3′ |
| Probe 1lnsertG | 5′-TGC ACC TGA CTC CTG G-3′ |
| Probe 1lnsertC | 5′-TGC ACC TGA CTC CTG C-3′ |
| Internal standard20-mers | 5′-CAT GGT GCA CCT GAC TCC TG-3′ |
aThe abbreviations in the table correspond to the following modifications: 3BioTEG, a biotin modifier with a tetraethyleneglycol (TEG) spacer on the 3′-end of the DNA; 5Phos, phosphorylation of the 5′-end of the DNA. These are the same notations used by the DNA supplier (Integrated DNA Technologies, Coralville, IA, USA). The underlined bases are paired at 3′-nick site.