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. 2009 Oct 23;38(1):e3. doi: 10.1093/nar/gkp841

Figure 2.

Figure 2.

Gymnotic delivery and silencing is observed in many cell lines. (A) Silencing of Bcl-2 protein expression in the 591.8, 1036 and 1000.36 melanoma cell lines by 2.5 and 5 µM SPC2996. Cells were treated for 10 days by gymnotic delivery before western blotting was performed. C, untreated control. (B) Separate experiments were performed to examine silencing of Bcl-2 protein expression in 591.8, 1036 and 1000.36 melanoma cell lines by 5 µM SPC2996 and the scrambled control oligomer SPC3046. Cells were treated for 6 days by gymnotic delivery before western blotting was performed. (C) Top: gymnotic delivery (10 days) and differential silencing of Bcl-2 protein expression in HT1080 fibrosarcoma cells by SPC2996. To the right, separate experiments were performed to examine gymnotic silencing of Bcl-2 (6 days) by SPC2996 and the scrambled control oligomer SPC3046; bottom: gymnotic delivery and silencing (10 days) of Bcl-2 protein expression in LNCaP cells, which can be accomplished with minimum toxicity, as compared with lipofection. (D) In vitro effects of the gymnotic delivery of SPC2996 on the expression of the Bcl-2 mRNA in Namalwa Burkitt’s lymphoma cells. qPCR analysis of Bcl-2 mRNA expression was performed after exposure to SPC2996 or SPC3088, the control oligomer (10 µM, final concentrations) for the indicated times. qPCR values represent the mean ± SD (n = 3).