Figure 4.

Co-localization of LNA oligonucleotides with gw182, a GW/P body marker. Confocal microscopic imaging of Huh-7 cells after gymnotic delivery of either a 12-mer (SPC5024) or a 16-mer (SPC4007) anti-ApoB 5′-FAM-3′, 5′-end-capped LNA phosphorothioate gap-mer. After 1 day (A) or 3 days (B) of delivery, cells were fixed and stained with an anti-gw182 primary antibody and visualized with a rhodamine-conjugated secondary antibody. After image merging, the oligomers were observed co-localizing with the GW/P body marker. The 12-mer accumulated more rapidly than the 16-mer after 1 day, but after 3 days of gymnotic delivery, the accumulation in the GW/P bodies are approximately equivalent.