Figure 9.

Illustrative schematic of the structure of four variants. Each exon is targeted by one exon–probeset. If differential splicing is predicted for exon 2, RT-PCR primers may be designed matching flanking exons 1 and 3. The resulting short gel product would show the expression of transcript A, while the long product would show the total expression of the two transcripts C and D. Any transcripts that do not include the exons matching the primers, in this case transcript B, are not targeted by RT-PCR.