Figure 2.

Secreted RNAs were sequestered in phospholipid vesicles. (A) Untreated CM or CM pretreated with SDS-based lysis buffer, cyclodextrin or phospholipase A2 was incubated with RNase A. After incubation, the CMs were extracted for RNA and the RNAs were resolved on a 15% TBE–urea gel. (B) RNA was extracted from CM without RNase inhibitor (lane 2), with RNase inhibitor (lane 3) or CM pretreated with cyclodextrin (lane 4), SDS-based lysis buffer (lane 5) or phospholipase A2 (lane 6).