Table 3.
Predicted location of HuD-binding motifs in selected known and new HuD targets
| mRNA | Motif 1 | Motif 2 | Motif 3 |
|---|---|---|---|
| Known HuD targets | |||
| GAP-43 | 128 332 | – | – |
| MARCKS | 518 522 526 530 534 538 542 546 | 151 158 275 276 277 278 279 | 111 559 560 |
| Neuroserpin | 1801 1803 1805 1807 1809 1811 1813 1815 1817 1819 1821 1823 1825 1827 1829 1831 1833 1835 1837 1839 1841 1843 | 2073 | 615 |
| N-myc | – | – | 244 245 369 |
| p21 waf1 | 133 1211 | – | – |
| tau | – | – | – |
| VEGF | 939 1261 | – | 397 |
| p27 5UTR | 225 233 306 | 436 | – |
| p27 3UTR | – | 878 879 880 881 882 883 884 885 886 887 888 889 | 475 597 891 |
| New HuD targets | |||
| eIF4e | – | 563 657 | – |
| HuR | – | 1190 1199 | – |
| HuB | – | 456 | – |
| Fkbp1a | 976 | – | – |
| PPP1cb | 985 | – | – |
| PPP3ca | – | 15 | – |
| CaMkinIIα | 1475 1483 1485 1487 1491 1497 1512 3032 3034 3036 3038 3040 3042 3044 3046 3048 3050 3052 3054 3056 3058 3060 3062 3064 3066 3068 3070 | 3294 3298 3302 3306 3312 | – |
| Homer1 | – | – | 754 |
| Rab1 | 891 1247 1249 | – | – |
| N-CAM1 | 75 77 79 81 83 | 744 1108 1109 1317 1334 1351 1352 1353 1354 1355 1356 1357 1358 1359 2833 2837 | 2705 |
| Musashi 2 | 274 276 280 | 253 416 421 422 426 456 461 1558 1563 1570 1575 1764 1765 2258 2263 2760 3632 3638 3889 4248 4253 | 1141 1580 1767 1768 1871 1872 2955 2956 3642 4082 4083 |
The predicted location of each motif in the 3′ UTR of selected HuD targets. As described in the ‘Materials and Methods’ section, Pearl scripts were used to search for the location of each motif, allowing one mismatch in each consensus sequence.