Figure 6.

Effect of the flanking TA dinucleotide on transposon excision. (A) Kinetic analysis of a transposition reaction was performed as described for Figure 5 using a donor plasmid (pRC824) in which the flanking 5′-TA dinucleotide at each end of the transposon was replaced by 5′-TG. (B and C) The effect of the 5′-dinucleotide on the rate of first-strand cleavage and excision. The gel in (A) of this figure and the gel shown in the right panel of Figure 5A were quantified as described in Figure 4.