Figure 3.

G-quadruplex formation in dsDNA derived from the C-MYC, NRAS gene examined by gel electrophoretic mobility shift. DNAs, including the control that does not form G-quadruplex, were subjected to heat denaturation/renaturation in 150 mM K⁺ or Li⁺ solution containing 40% (w/v) PEG 200, then followed by incubation in the presence or absence of single-stranded DNA binding protein (SSB) before native gel electrophoresis. The gel was stained with ethidium bromide (EB). The drawing at the right side shows schematic illustration of the structure associated with the indicated DNA band.