Figure 2.

Concentration–response effect of ZK200775 on kainate-induced currents in neonatal rat hippocampal neurons. Current amplitudes were leak-subtracted, normalized to control, and plotted against antagonist concentration. Data represent means ± SEM from 5–13 determinations. (Lower Inset) Original current traces demonstrating effect of ZK200775, 0.01, 0.1, and 1 μM on kainate (100 μM) induced currents in a single cell. (Vertical scale bar = 100 pA; horizontal scale bar = 10 s.) (Upper Inset) Schild analysis of the antagonism by ZK200775 derived from partial concentration–response curves for kainate in the presence of increasing concentrations of ZK200775 (0.03, 0.1, 0.3, and 1 μM). Current amplitudes were leak-subtracted and related to the maximal current obtained at 10 mM kainate in the absence of the antagonist.