Table 3.

Effect of ZK200775 on neuronal loss in the hippocampal CA1 subfield and locomotor activity in gerbils subjected to bilateral occlusion of common carotid arteries (CCAO) for 4 min

Surgery	Vehicle	ZK200775
Neuronal loss score in hippocampal CA1 subfield
CCAO	5.97 (17)	4.89* (13)
Exploratory activity (counts)
CCAO	12804  ± 1307	7296  ± 1200**
Sham	7382  ± 1274 (10)	5679  ± 603 (10)

Mongolian gerbils (Møllegaard) were subjected to i.p. administration of ZK200775 in the dose of 10 mg/kg 30, 90, 150, and 210 min after CCAO. Body temperature was maintained at 37.5 ± 0.5°C. Locomotor activity was measured 3 days after CCAO for 30 min. The extent of degeneration in the hippocampus was assessed 24 h later in cresyl violet and hematoxilin/eosin-stained coronal sections of the entire brain and blindly scored on each site from 0 (no cell loss) to 3 (total cell loss). The maximal damage score for both hippocampal CA1 subfields was 6.0 

^*P < 0.05 (Mann–Whitney U test); **P < 0.01 (Student’s t test) vs vehicle-treated gerbils.