Fig. 2.

Effects of the fatty acids on the expression level of RABGGTA and the role of RABGGTA in cytotoxicity. HepG2 cells were exposed to 0.7 mM palmitate or oleate for 24 hours (a). After treatment, the cells were harvested, and RT-PCR analysis was performed to detect the mRNA levels of RABGGTA (a). Reverse transfection of suspended HepG2 cells were performed with scrambled siRNA (whit bar, negative control) or siRNA of RABGGTA (gray bars, siRABGGTA) for 24 h and the transfected cells were then cultured in 0.7 mM palmitate or oleate for another 24 h (b). Cells were then harvested, and the LDH release was assayed (b). Data expressed as average of nine samples ±SD from three independent experiments. Student's t-test was used to analyze the differences between treatment groups. Significantly higher (*) or lower (**) than negative control, i.e. scrambled siRNA; P < 0.01.