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. 2009 Oct 8;19(1):99–110. doi: 10.1093/hmg/ddp470

Figure 2.

Figure 2.

Mapping origins of replication within the FRA3B. (A) Location of the FRA3B region within the FHIT gene, and of the 50 kb region of interest within FRA3B containing the sequences encompassed by the HP2A probe. All of the chromosomal coordinates correspond to NCBI Build 35. (B) Location of primers used for the real-time PCR for (a) FRA3B ori 1, (b) ori 2 and (c) ori 3. (C) Microarray analysis of nascent strand DNA in untreated cells. The ‘probe location’ bar shows the location of probes included on the microarray. The nascent strand DNA abundance data (log2 ratio of nascent strand DNA signal over G1 DNA signal) obtained for each of the four sets of probes represented on the microarray (probes representing the forward and reverse strands are present in duplicate: FWD1, FWD2, REV1, REV2), and the location of the mapped origins are shown across a 50 kb region within FRA3B for two independent experiments.