Figure 1.

(A) Nucleotide and amino acid sequences of a cDNA encoding the 5′ end of an ovarian form of TH mRNA type 1 isolated by RT-PCR from rhesus monkey (Rh, M. mulatta) ovaries. The ovarian sequence differs from that of testes (16), only by a silent nucleotide substitution (G instead of A, denoted by an underlined bold italics letter). The sequence is compared with that isolated from adrenal gland of M. mulatta (16). Nucleotides or amino acids in the ovarian sequence that are similar to those in the adrenal sequence are shown as dashes. Letters in bold denote nucleotide or amino acid substitutions in the ovarian sequence relative to the adrenal sequence. A three-nucleotide addition that results in an extra amino acid (Ala) is shown by a dotted line. The sequence of adrenal TH mRNA is identical to that of Macaca fuscata (26), with the exception of two conservative nucleotide substitutions (asterisks on the adrenal sequence). The latter substitution does not occur in the ovarian TH mRNA form. Boxed regions correspond to the deoxynucleotides sequences used for PCR amplification. (B) Nucleotide and amino acid sequence of a cDNA encoding the mid-portion of DBH mRNA isolated from the rhesus monkey ovary by RT-PCR. The sequence, identical to that isolated from the monkey adrenal, is compared with that of human DBH (18). For other details, see A.