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. Author manuscript; available in PMC: 2010 Dec 29.
Published in final edited form as: Neuroscience. 2009 Sep 22;164(4):1854–1866. doi: 10.1016/j.neuroscience.2009.09.037

Figure 10.

Figure 10

GABA (0.5 mM) induced inward current recorded from SGN (holding potential -70 mV). (A) GABA-induced current at C7 has a fast onset followed by a slower decay after 1-2 seconds. (B) GABA induced current blocked by bicuculline (BIC, 0.5 mM), a GABA-A receptor antagonist. (C) GABA induced current quickly recovered after BIC was washed out. (D) In the control neurotrophin free cultures (CNT), GABA-induced currents were recorded from 60% of short term SGN cultures (<C3) and 40% of long term SGN cultures (>C7). Adding NT-3 to the cultures increased the number of GABA positive SGNs to nearly 100%; adding BDNF only slightly increased the percentage of GABA-positive SGN. (E) Amplitude of GABA-induced currents recorded from long term SGN cultured in NT-3 was greater than in those recorded from SGN cultured with BDNF or neurotrophin free medium.