Figure 7.

Ascl1a knockdown prevents induction of endogenous α1T and pax6. Retinas from −1016 transgenic fish were injured and electroporated with lissamine-labeled MOs on day 0 and harvested on day 4. a–f, α1T expression detected by ISH is shown as a dark brown deposit (a, d), native GFP expression is shown in green (b, e), and lissamine-labeled MO is shown in red (b, e). The injury site is marked by an asterisk. a–c, Control MO treatment does not affect endogenous or transgene α1T induction (arrows). d–f, Ascl1a knockdown prevents endogenous and transgene α1T induction. Note the lack of α1T and GFP expression between the asterisk and the arrows, where the retina is treated with MO. Where the retina did not receive MO, α1T and GFP are expressed (arrows). g–l, Pax6 expression detected by ISH is shown as a blue/purple deposit, native GFP expression is shown in green, and MO is shown in red. g–i, Control MO treatment does not affect pax6 induction at 4 dpi. Arrows indicate pax6⁺/MO⁺/GFP⁺ Müller glia. j–l, Ascl1a MO treatment prevents pax6 induction. Arrowheads indicate MO⁺/pax6⁻ cells. Rare GFP⁺ cells are sometimes present but were not treated with MO (arrow).