Figure 8.

Ascl1a is required for proliferation of Müller glia. Morpholinos were electroporated into injured −1016 retinas on day 0 and fish were housed in BrdU-treated water from 36 to 60 h after injury to label dividing cells. The fish recovered until day 10 when eyes were harvested. The control morpholino does not prevent treated cells from labeling with BrdU (arrowheads; top 3 control panels). Morpholinos targeting ascl1a (ascl1a and ascl1a^5′UTR) prevent cells from labeling with BrdU (arrows; ascl1a and ascl1a^5′UTR panels). Some cells are able to proliferate but were not treated with morpholino (arrowheads; ascl1a and ascl1a^5′UTR panels). GCL, Ganglion cell layer.