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The free energy landscapes (in kBT) for the RNA three-way junction at different [Na+] (A–C) and [Mg2+] (with 0.05 M Na+ background, D–F) concentrations. The x axis (θ) is the interaxis angles between 18-bp helix and 15-bp helix. The y axis is the free energy relative to the fully unfolded state at θ = 120°. For the folded state θ = 60°, the nonelectrostatic free energy (for the coaxial-stacking) is included in the total free energy.
