Figure 5.

Shown is a loop connecting two alpha-helices from Target 432, with the starting model (teal), a top prediction (blue), and the deposited PDB structure (3DAI, purple). Solid molecular surfaces of nearby symmetry mates of the PDB structure are colored dark grey, which clash with both the starting model and prediction. The 2F_o − F_c iso-contour at 3σ, shown in wireframe, emphasizes that the loop and side chains are well resolved by the experimental data. The loop conformation may well be influenced by interactions with symmetry mates. Although it is a challenge to take into account crystal-packing effects, this may be a limiting factor in the agreement of CASP entries with experiment as measured by MR statistics.